What is 50X TAE Buffer used for?

50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis.

How do I dilute 50X TAE Buffer for use?

To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis.

What is the shelf life of 50X TAE Buffer?

The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure.

Is 50X TAE Buffer compatible with agarose gel electrophoresis?

Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands.

Can I use 50X TAE Buffer for RNA electrophoresis?

Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results.

What are the components of 50X TAE Buffer?

50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments.

Is 50X TAE Buffer necessary for DNA gel extraction?

50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures.

How should I store 50X TAE Buffer?

To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis.

What are some common applications for using 50X TAE Buffer?

50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results.

Where can I buy 50X TAE Buffer, 100 mL?

You can buy 50X TAE Buffer, 100 mL on Ubuy in Panama. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.

¿Cómo comprar 50X TAE Buffer, 100 mL en línea desde Ubuy?

Es fácil comprar 50X TAE Buffer, 100 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está 50X TAE Buffer, 100 mL disponible para comprar en línea en Panama?

Sí, en Ubuy Panama, este producto está disponible para que lo compre a un precio razonable.. El 50X TAE Buffer, 100 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.

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50X TAE Buffer, 100 mL

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What Stands Out

Concentrated Solution

This 50X TAE buffer provides a highly concentrated formulation, ensuring ease of use and longer shelf life, making it ideal for high-throughput laboratories and educational institutions.

Versatile Applications

Designed for various molecular biology techniques, this buffer ensures optimal performance for DNA electrophoresis and other applications, effectively meeting diverse research needs in a single product.

Precise Composition

Formulated with high purity components, this TAE buffer guarantees reproducibility and reliability in experiments, catering to both novice and expert researchers who demand consistent results.

Detalles de producto

Item Form	Gel
Item Weight	4 ounces (113.4 grams)
Volume	100 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.171
Brand	eCon Lab Supply
Item Volume	100 ml

Who Should Buy?

Molecular Biologists
Ideal for electrophoresis applications, aiding in the separation and analysis of nucleic acids in a lab environment.
Academic Researchers
Perfect for students and researchers conducting experiments that require buffer solutions in their molecular biology studies.
DNA & RNA Studies
Essential for protocols involving DNA and RNA handling, ensuring stability and integrity during electrophoresis experiments.

Clinical Laboratories
Not suitable for clinical environments where strict regulatory compliance and high precision are required in diagnostics.

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50X TAE Buffer, 100 mL

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Preguntas y respuestas de los clientes

Pregunta: ¿Cómo comprar 50X TAE Buffer, 100 mL en línea desde Ubuy?

Respuesta: Es fácil comprar 50X TAE Buffer, 100 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está 50X TAE Buffer, 100 mL disponible para comprar en línea en Panama?

Respuesta: Sí, en Ubuy Panama, este producto está disponible para que lo compre a un precio razonable.. El 50X TAE Buffer, 100 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

eCon Lab Supply Buffers Editorial Review

50X TAE Buffer, 100 mL is a high-quality gel designed for molecular biology applications. Manufactured by Ciro Mfg under the eCon Lab Supply brand, this product comes in a 100 mL bottle, making it convenient for various laboratory uses. The buffer is essential for electrophoresis and gel preparation, providing reliable performance across experiments. Its formulation ensures optimal separation and resolution of DNA fragments. Users appreciate its consistent quality and ease of use, making it a go-to choice for both seasoned professionals and laboratory novices alike.

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High-quality gel for molecular biology applications
Comes in a convenient 100 mL bottle
Essential for electrophoresis and gel preparation
Provides reliable performance in experiments
Ensures optimal separation of DNA fragments

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
Inhibits nucleases to protect DNA integrity during experiments.
Powers efficient running of both genomic and supercoiled DNA.
Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
Manufactured under strict quality control in an ISO certified facility.
Ideal for research purposes, compliant with Good Manufacturing Practices.

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50X TAE Buffer, 100 mL

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eCon Lab Supply Buffers Editorial Review

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